Human/Mouse IGF-I R/IGF1R Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-305-NA
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Cell Culture, Functional Assay, Immunohistochemistry, Immunohistochemistry-Paraffin, Luciferase Reporter Assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Recombinant Human IGF-I R/IGF1R extracellular domain
Accession # P08069
Accession # P08069
Specificity
Detects human and mouse IGF-I R/IGF1R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Mouse IGF-I R/IGF1R Antibody
IGF-I R/IGF1R in MCF‑7 and HDLM-2 Human Cell Lines.
IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and HDLM-2 human Hodgkin's lymphoma cell line (negative control, right panel) using Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslipsand Fluorescent ICC Staining of Non-adherent Cells.
IGF-I R/IGF1R in Mouse Embryo.
IGF-I R/IGF1R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IGF-I R/IGF1R in Human Placenta.
IGF-I R/IGF1R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human/Mouse IGF-I R/IGF1R Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) and immersion fixed frozen sections of mouse embryo
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) and immersion fixed frozen sections of mouse embryo
Western Blot
0.1 µg/mL
Sample: Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) and Recombinant Mouse IGF-I R/IGF1R (Catalog # 6630-GR).
Sample: Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) and Recombinant Mouse IGF-I R/IGF1R (Catalog # 6630-GR).
Neutralization
Measured by its ability to neutralize IGF-I-induced proliferation in the MCF-7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL
Recombinant
Human IGF-I/IGF-1 (Catalog # 291-G1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using AF-305-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I R/IGF1R
References
- Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
Long Name
Insulin-like Growth Factor I Receptor
Alternate Names
CD221, IGF-1R, IGF-IR, IGF1R, IGFIR
Entrez Gene IDs
3480 (Human)
Gene Symbol
IGF1R
UniProt
Additional IGF-I R/IGF1R Products
Product Documents for Human/Mouse IGF-I R/IGF1R Antibody
Product Specific Notices for Human/Mouse IGF-I R/IGF1R Antibody
For research use only
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