Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6434
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human KDM4A
Lys386-Ser514
Accession # AAH02558
Lys386-Ser514
Accession # AAH02558
Specificity
Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody
Detection of Human and Mouse Lysine (K)‑specific Demethylase 4A/KDM4A by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 4A/KDM4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6434) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 4A/KDM4A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
KDM4A in BG01V Human Cell Line.
KDM4A was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Human/Mouse KDM4A Affinity-purified Polyclonal Antibody (Catalog # AF6434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed endoderm-differentiated BG01V human embryonic stem cells
Sample: Immersion fixed endoderm-differentiated BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, THP‑1 human acute monocytic leukemia cell line, and mouse embryo tissue
Sample: HeLa human cervical epithelial carcinoma cell line, THP‑1 human acute monocytic leukemia cell line, and mouse embryo tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysine (K)-specific Demethylase 4A/KDM4A
Alternate Names
JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A
Gene Symbol
KDM4A
UniProt
Additional Lysine (K)-specific Demethylase 4A/KDM4A Products
Product Documents for Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody
Product Specific Notices for Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody
For research use only
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