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Human/Mouse Myeloperoxidase/MPO Antibody Best Seller

R&D Systems, part of Bio-Techne | Catalog # AF3667

R&D Systems, part of Bio-Techne
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AF3667
AF3667-SP

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Hamster, Primate, Transgenic Mouse, Xenograft

Applications

Validated:

Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Cited:

ELISA Capture, ELISA Development, Flow Cytometry, Functional Assay, IHC/IF, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247

Specificity

Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Myeloperoxidase/MPO Antibody

Detection of Human/Mouse Myeloperoxidase/MPO antibody by Western Blot.

Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot.

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Myeloperoxidase/MPO antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

Myeloperoxidase/MPO in Mouse Splenocytes.

Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
For human Immunocytochemistry samples, we recommend Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (MAB3174).
Myeloperoxidase/MPO antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P).

Myeloperoxidase/MPO in Mouse Spleen Tissue.

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.
For human Immunohistochemistry samples, we recommend Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (MAB3174).

Applications for Human/Mouse Myeloperoxidase/MPO Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
For human Immunocytochemistry samples, we recommend Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174)

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

For human Immunohistochemistry samples, we recommend Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174)

Simple Western

5 µg/mL
Sample: Mouse spleen tissue

Western Blot

0.5 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue

Reviewed Applications

Read 7 reviews rated 4.9 using AF3667 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta-subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta-subunits and two alpha-subunits that are also disulfide-linked through the two alpha-subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Alternate Names

MPO

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Gene Symbol

MPO

UniProt

Additional Myeloperoxidase/MPO Products

Product Documents for Human/Mouse Myeloperoxidase/MPO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Myeloperoxidase/MPO Antibody

For research use only

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