Human/Mouse Myogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6686
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 671038
Product Specifications
Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Ser124-Asn224
Accession # P15173
Specificity
Detects human Myogenin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Myogenin Antibody
Myogenin in C2C12 Mouse Myoblast Cells.
Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to select differentiating nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Myogenin in C2C12 cells by Flow Cytometry.
C2C12 cells were stained with Mouse Anti-Human/Mouse Myogenin Monoclonal Antibody (Catalog # MAB6686, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Myogenin Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line
Sample: C2C12 mouse myoblast cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion-fixed differentiated C2C12 mouse myoblast cells
Sample: Immersion-fixed differentiated C2C12 mouse myoblast cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myogenin
Alternate Names
BHLHC3, MYF4, MYOG
Gene Symbol
MYOG
UniProt
Additional Myogenin Products
Product Documents for Human/Mouse Myogenin Antibody
Product Specific Notices for Human/Mouse Myogenin Antibody
For research use only
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