Human/Mouse Orexin A/Hypocretin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB763
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat, Camelid - Vicugna pacos (Alpaca), Canine
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 97505
Product Specifications
Immunogen
KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612
Accession # O43612
Specificity
Detects human and mouse Orexin A/Hypocretin‑1. In dot blots, no cross-reactivity with recombinant human Orexin B is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Orexin A/Hypocretin-1 Antibody
Orexin A/Hypocretin-1 in Mouse Hypothalamus.
Orexin A/Hypocretin-1 was detected in immersion fixed paraffin-embedded sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin-1 Monoclonal Antibody (Catalog # MAB763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse Orexin A/Hypocretin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Hypothalamus
Sample: Immersion fixed paraffin-embedded sections of Mouse Hypothalamus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Orexin A/Hypocretin-1
References
- Sakurai, T. et al. (1998) Cell 92:573.
Alternate Names
HCRT, Hcrt1, Hypocretin-1, Hypocretin1
Gene Symbol
HCRT
UniProt
Additional Orexin A/Hypocretin-1 Products
Product Documents for Human/Mouse Orexin A/Hypocretin-1 Antibody
Product Specific Notices for Human/Mouse Orexin A/Hypocretin-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...