Human/Mouse Park7/DJ-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3668

R&D Systems, part of Bio-Techne
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AF3668
AF3668-SP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Park7/DJ-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Park7/DJ-1
Ala2-Asp189
Accession # Q99LX0

Specificity

Detects human and mouse Park7/DJ-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Park7/DJ-1 Antibody

Detection of Human/Mouse Park7/DJ-1 antibody by Western Blot.

Detection of Human/Mouse Park7/DJ-1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Park7/DJ-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Park7/DJ-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Park7/DJ‑1 in HeLa Human Cell Line.

Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Park7/DJ-1 in C2C12 Mouse Cell Line.

Park7/DJ-1 was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Applications for Human/Mouse Park7/DJ-1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line

Knockout Validated

Park7/DJ‑1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Park7/DJ‑1 knockout HEK293T cell line.

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease. Human and mouse Park7 share 92% amino acid sequence identity.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Entrez Gene IDs

11315 (Human); 57320 (Mouse); 117287 (Rat)

Gene Symbol

PARK7

UniProt

Q99LX0

Additional Park7/DJ-1 Products

Product Documents for Human/Mouse Park7/DJ-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Park7/DJ-1 Antibody

For research use only

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