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Human/Mouse PARP Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF600

R&D Systems, part of Bio-Techne
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BAF600

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse PARP
Val71-Pro329

Specificity

Detects human and mouse PARP in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse PARP Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PARP
Recombinant Mouse PARP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARP

PARP, Poly [ADP-ribose] polymerase 1 (PARP1), is a widely expressed component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. PARP expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult. PARP is upregulated in B cells that have been induced to switch to various Ig isotypes. PARP interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Entrez Gene IDs

142 (Human); 11545 (Mouse)

Gene Symbol

PARP1

Additional PARP Products

Product Documents for Human/Mouse PARP Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse PARP Biotinylated Antibody

For research use only

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