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Human/Mouse/Rat ATG5 Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC5294T

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 603813

Product Specifications

Immunogen

E. coli-derived recombinant human ATG5
Asn99-Thr193
Accession # Q9H1Y0

Specificity

Detects human, mouse, and rat ATG5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat ATG5 Alexa Fluor® 594-conjugated Antibody

Detection of ATG5 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of ATG5 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat ATG5 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC5294T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat ATG5 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.

Long Name

ATG5 Autophagy Related 5 Homolog

Alternate Names

APG5, ASP

Entrez Gene IDs

9474 (Human); 11793 (Mouse)

Gene Symbol

ATG5

UniProt

Additional ATG5 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat ATG5 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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