Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human and Rat ATG5 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG5 at approximately 51 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat ATG5 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded human small intestine

Simple Western

25 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑12 rat adrenal pheochromocytoma cell line

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB5294 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.

Long Name

ATG5 Autophagy Related 5 Homolog

Alternate Names

APG5, ASP

Entrez Gene IDs

9474 (Human); 11793 (Mouse)

Gene Symbol

ATG5

UniProt

Q9H1Y0 (Human)

Additional ATG5 Products

Product Documents for Human/Mouse/Rat ATG5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat ATG5 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat ATG5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5294

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat ATG5 Antibody

Detection of Human, Mouse, and Rat ATG5 by Western Blot.

Detection of Human and Rat ATG5 by Simple WesternTM.