Human/Mouse/Rat Beclin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5295
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 669922
Product Specifications
Immunogen
E.coli-derived recombinant human Beclin 1
Met12-Gln239
Accession # Q14457
Met12-Gln239
Accession # Q14457
Specificity
Detects human Beclin 1 in direct ELISAs. Detects human, mouse, and rat Beclin 1 in Western blots. In direct ELISAs,no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Beclin 1 Antibody
Detection of Human, Mouse, and Rat Beclin 1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Beclin 1 Monoclonal Antibody (Catalog # MAB5295) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Beclin 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Detection of Human Beclin 1 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Detection of Mouse Beclin 1 by Simple WesternTM.
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Beclin 1 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney
Sample: Immersion fixed paraffin-embedded sections of Human Kidney
Simple Western
1 µg/mL
Sample: Human brain (cerebellum) tissue and mouse brain tissue
Sample: Human brain (cerebellum) tissue and mouse brain tissue
Western Blot
0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line
Sample: A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line
Reviewed Applications
Read 1 review rated 5 using MAB5295 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Beclin 1
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Gene Symbol
BECN1
UniProt
Additional Beclin 1 Products
Product Documents for Human/Mouse/Rat Beclin 1 Antibody
Product Specific Notices for Human/Mouse/Rat Beclin 1 Antibody
For research use only
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