Human/Mouse/Rat Casein Kinase 1 delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4568
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CK1 delta
Arg316-Arg415
Accession # P48730
Arg316-Arg415
Accession # P48730
Specificity
Detects endogenous human, mouse and rat CK1 delta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha or rhCK1 gamma; reactivity with CK1 epsilon is unknown.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 delta Antibody
Detection of Human/Mouse/Rat Casein Kinase 1 delta by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1d at approximately 49 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Casein Kinase 1 delta in C2C12 Mouse Cell Line.
Casein Kinase 1d was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Casein Kinase 1 delta Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line
Sample: Immersion fixed C2C12 mouse myoblast cell line
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and Y3-Ag rat myeloid cell line
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and Y3-Ag rat myeloid cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 delta
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma1, gamma2, gamma3 , delta and epsilon) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 delta phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity.
Alternate Names
CSNK1D, HCKID
Gene Symbol
CSNK1D
UniProt
Additional Casein Kinase 1 delta Products
Product Documents for Human/Mouse/Rat Casein Kinase 1 delta Antibody
Product Specific Notices for Human/Mouse/Rat Casein Kinase 1 delta Antibody
For research use only
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