Human/Mouse/Rat Casein Kinase 1 epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4567
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CK1 epsilon
Met317-Lys416
Accession # P49674
Met317-Lys416
Accession # P49674
Specificity
Detects endogenous human, mouse and rat CK1 epsilon in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha, CK1 delta, or CK1 gamma.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody
Detection of Human/Mouse/Rat Casein Kinase 1 epsilon by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4567) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line, PC-12 rat adrenal pheochromocytoma cell line
Sample: NRK rat normal kidney cell, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line, PC-12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 epsilon
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma1, gamma2, gamma3 , delta and epsilon) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 epsilon phosphorylates all three mammalian PER proteins, as well as BMAL1 and CRY, thereby modulating the activities of these circadian clock regulators.
Alternate Names
CSNK1E
Gene Symbol
CSNK1E
UniProt
Additional Casein Kinase 1 epsilon Products
Product Documents for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody
Product Specific Notices for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody
For research use only
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