Human/Mouse/Rat Caveolin-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5788
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Caveolin-2
Met1-Lys86
Accession # P51636
Met1-Lys86
Accession # P51636
Specificity
Caveolin-2 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse, and rat Caveolin-2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Caveolin-2 Biotinylated Antibody
Caveolin‑2 in HeLa Human Cell Line.
Caveolin-2 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Caveolin-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL overnight at 4° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to caveolae.
Caveolin-2 in L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line.
Caveolin-2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Applications for Human/Mouse/Rat Caveolin-2 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-2
Alternate Names
CAV2, Caveolin2
Gene Symbol
CAV2
UniProt
Additional Caveolin-2 Products
Product Documents for Human/Mouse/Rat Caveolin-2 Biotinylated Antibody
Product Specific Notices for Human/Mouse/Rat Caveolin-2 Biotinylated Antibody
For research use only
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