Human/Mouse/Rat Cubilin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3700
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cubilin
Ser36-Gly126
Accession # O60494
Ser36-Gly126
Accession # O60494
Specificity
Detects human Cubilin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Cubilin Antibody
Detection of Human, Mouse, and Rat Cubilin by Western Blot.
Western blot shows lysates of human, mouse, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody HAF016). A specific band was detected for Cubilin at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cubilin in Human Kidney Tissue.
Cubilin was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Sheep Anti-Human/Mouse/Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (brown; (VC006) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Cubilin by Simple WesternTM.
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Cubilin at approximately 500 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human/Mouse/Rat Cubilin Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue
Simple Western
10 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Western Blot
1 µg/mL
Sample: Human, mouse, and rat kidney tissue
Sample: Human, mouse, and rat kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF3700 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cubilin
Cubilin is a 460 kDa protein that is a component of a multiligand endocytic receptor complex, which also contains amnionless, megalin and LRP2. The receptor complex is expressed in several absorptive epithelia, including kidney proximal tubule, yolk sac, and the small intestine. Human Cubilin is synthesized as a 3623 residue precursor with a signal peptide, an amino-terminal domain, eight EGF-like repeats and 27 Cub domains. Co-expression with amnionless is required for the membrane anchoring, ER export and internalizing action of Cubilin. Within the region used as the immunogen, human, mouse and rat Cubilin share approximately 76% amino acid sequence homology.
Alternate Names
CUBN, Cubulin, gp280, IFCR, MGA1
Gene Symbol
CUBN
UniProt
Additional Cubilin Products
Product Documents for Human/Mouse/Rat Cubilin Antibody
Product Specific Notices for Human/Mouse/Rat Cubilin Antibody
For research use only
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