Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5169
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase
Met1-Leu434
Accession # P09104
Met1-Leu434
Accession # P09104
Specificity
Detects human and mouse Enolase 2/Neuron-specific Enolase in direct ELISAs. Detects human, mouse, and rat Enolase 2/Neuron-specific Enolase in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
Detection of Human/Mouse Enolase 2 by Western Blot.
Western blot shows lysates of human brain, mouse brain tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Rat Enolase 2/Neuron‑specific Enolase by Western Blot.
Western blot shows lysates of rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Enolase 2 in Human Brain.
Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Simple Western
10 µg/mL
Sample: BG01V human embryonic stem cells
Sample: BG01V human embryonic stem cells
Western Blot
0.2-1 µg/mL
Sample: Human brain, mouse brain tissue, BG01V human embryonic stem cells, rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue
Sample: Human brain, mouse brain tissue, BG01V human embryonic stem cells, rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue
Reviewed Applications
Read 4 reviews rated 4 using AF5169 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Enolase 2/Neuron-specific Enolase
Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
UniProt
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
Product Specific Notices for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
For research use only
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