Human/Mouse/Rat FABP1/L-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7009
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse FABP1/L-FABP
Met1-Ile127
Accession # P12710
Met1-Ile127
Accession # P12710
Specificity
Detects human, mouse, and rat FABP1/L-FABP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat FABP1/L-FABP Antibody
Detection of Human, Mouse, and Rat FABP1/ L‑FABP by Western Blot.
Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP1/L‑FABP in Mouse Intestine.
FABP1/L-FABP was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Human/Mouse/Rat FABP1/ L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7009) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to apical portion of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human FABP1/L-FABP by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated) using 1 µg/mL of Sheep Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7009) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat FABP1/L-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Simple Western
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
0.1 µg/mL
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
FABP1 (fatty acid binding protein-1; also Liver-type fatty acid binding protein/L-FABP and 14 kDa Selenium-binding protein) is a 14-15 kDa intracellular member of the fatty acid binding protein family, calycin superfamily of molecules. It is expressed by intestinal epithelium and hepatocytes, and is known to bind both cholesterol and long-chain fatty acids in the cytosol. As fatty acids diffuse across the plasma membrane, they are sequestered by FABP1, facilitating fatty acid diffusion into the cell. FABP1 also binds bile acids and cholesterol, and transports cholesterol from one membrane compartment to another. Mouse FABP1 is 127 amino acids (aa) in length. It is a two beta-sheet molecule that contains an acetylated initiating methionine. Full-length mouse FABP1 shares 94% and 84% aa identity with rat and human FABP1, respectively.
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
UniProt
Additional FABP1/L-FABP Products
Product Documents for Human/Mouse/Rat FABP1/L-FABP Antibody
Product Specific Notices for Human/Mouse/Rat FABP1/L-FABP Antibody
For research use only
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