Human/Mouse/Rat FABP3/H-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1678
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Primate - Macaca fuscata (Japanese Macaque)
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP3/H‑FABP
Met1-Ala133
Accession # P05413
Met1-Ala133
Accession # P05413
Specificity
Detects human FABP3/H‑FABP in direct ELISAs and detects human, mouse, and rat FABP3/H-FABP in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) FABP4 and recombinant human (rh) FABP4 is observed, approximately 15% cross-reactivity with recombinant rat (rr) FABP1, rhFABP1, rhFABP2, rhFABP7, and rhFABP8 is observed, and less than 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat FABP3/H-FABP Antibody
Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot.
Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP3/H‑FABP in Human Heart.
FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat FABP3/H-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human heart and skeleton muscle
Sample: Immersion-fixed paraffin-embedded sections of human heart and skeleton muscle
Simple Western
5 µg/mL
Sample: Human heart tissue and mouse heart tissue
Sample: Human heart tissue and mouse heart tissue
Western Blot
0.5 µg/mL
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP3/H-FABP
Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.
Long Name
Fatty Acid-Binding Protein 3
Alternate Names
H-FABP, HFABP, MDGI
Entrez Gene IDs
2170 (Human)
Gene Symbol
FABP3
UniProt
Additional FABP3/H-FABP Products
Product Documents for Human/Mouse/Rat FABP3/H-FABP Antibody
Product Specific Notices for Human/Mouse/Rat FABP3/H-FABP Antibody
For research use only
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