Human/Mouse/Rat Fyn Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3574
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 361228
Product Specifications
Immunogen
E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241
Met1-Gly83
Accession # P06241
Specificity
Detects endogenous human, mouse and rat Fyn in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Fyn Antibody
Detection of Human/Mouse/Rat Fyn by Western Blot.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fyn at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Fyn in Human PBMCs.
Fyn was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat Fyn Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Western Blot
0.5 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line , C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: MOLT-4 human acute lymphoblastic leukemia cell line , C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fyn
Long Name
FYN oncogene related to SRC, FGR, YES
Alternate Names
SLK, SYN
Entrez Gene IDs
2534 (Human)
Gene Symbol
FYN
UniProt
Additional Fyn Products
Product Documents for Human/Mouse/Rat Fyn Antibody
Product Specific Notices for Human/Mouse/Rat Fyn Antibody
For research use only
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