Human/Mouse/Rat Gelsolin/GSN Antibody

Detection of Gelsolin/GSN in Human Renal Cell Carcinoma via seqIF™ staining on COMET™

Gelsolin/GSN Antibody was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Gelsolin/GSN by Simple Western^TM.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (U-87 MG) (NBP2-49844) and SK-Mel-28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Gelsolin/GSN at approximately 90 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.