Human/Mouse/Rat GLUD1/GLUD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8027
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 848209
Product Specifications
Immunogen
E. coli-derived recombinant human GLUD1/GLUD2
Ser54-Thr558
Accession # P49448
Ser54-Thr558
Accession # P49448
Specificity
Detects human, mouse and rat GLUD1/GLUD2 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat GLUD1/GLUD2 Antibody
Detection of Human, Mouse, and Rat GLUD2 by Western Blot.
Western blot shows lysates of human brain (cortex) tissue, human testis tissue, mouse brain (cortex) tissue, and rat brain (cortex) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GLUD2 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GLUD1/GLUD2 in Rat Brain.
GLUD1/GLUD2 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027) at 0.2 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human GLUD2 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.5 mg/mL. A specific band was detected for GLUD2 at approximately 58 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/RatGLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat GLUD1/GLUD2 Antibody
Application
Recommended Usage
Immunohistochemistry
0.2-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain
Sample: Perfusion fixed frozen sections of rat brain
Simple Western
2 µg/mL
Sample: Human brain (motor cortex) tissue
Sample: Human brain (motor cortex) tissue
Western Blot
0.2 µg/mL
Sample: Human brain (cortex) tissue, human testis tissue, mouse brain (cortex) tissue, and rat brain (cortex) tissue
Sample: Human brain (cortex) tissue, human testis tissue, mouse brain (cortex) tissue, and rat brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLUD1/GLUD2
Alternate Names
epididymis secretory sperm binding protein, epididymis tissue sperm binding protein Li 18mP, GDH, GDH1, GLUD, glutamate dehydrogenase (NAD(P)+), glutamate dehydrogenase 1, mitochondrial, hGDH1
UniProt
Additional GLUD1/GLUD2 Products
Product Documents for Human/Mouse/Rat GLUD1/GLUD2 Antibody
Product Specific Notices for Human/Mouse/Rat GLUD1/GLUD2 Antibody
For research use only
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