Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody

Western Blot Shows Human Glutathione Peroxidase 1/GPX1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 24 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Glutathione Peroxidase 1/GPX1 by Western Blot

Induction of oxidative stress defense during muscle mild mitochondrial stress. (A) Skeletal muscle mRNA expression in quadriceps muscle of oxidative stress defense markers of 24 wks old animals (n = 8–10). (B and C) Representative immunoblots of glutathione peroxidase 1 (GPX1) and GPX4 from of cropped gels using alphaTubulin as loading control (2 samples shown out of 4–5 analyzed per group). (D) Quantification of GPX1 and GPX4 protein expression in quadriceps muscle from 24 wks old mice (4–5 per group). (E) Skeletal muscle catalase activity of 24 wks old animals (n = 5–10). (F) Skeletal muscle total GPX activity of 24 wks old animals (n = 7–10). (G) Representative immunoblots of methylglyoxal advanced glycation end-products (AGEs) in quadriceps muscle from 24 wks old mice using alphaTubulin ( alphaTUB) as loading control (2 samples shown out of 4–5 analyzed per group). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; *p < 0.05; **p < 0.01; ***p < 0.001. (H) Graphical summary of Nrf2-dependent and Nrf2-independent adaptive cell-autonomous stress response pathways during respiratory uncoupling-induced muscle (mild) mitochondrial stress. This cartoon was created using Servier Medical Art (https://smart.servier.com). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-20901-4), licensed under a CC-BY license. Not internally tested by R&D Systems.