Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Intracellular Staining by Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 272536

View all GSK-3 beta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841

Specificity

Detects human, mouse, and rat GSK-3 beta in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Antibody

Detection of GSK-3 beta antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of GSK‑3 beta in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2506F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta) share 85% amino acid sequence identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development. Depending upon the substrate, phosphorylation may either promote activity (SIK-1; OGT; ZC3HAV1) or downregulate activity (SNAI1; BMAL1; MARK2).

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Entrez Gene IDs

2932 (Human); 56637 (Mouse); 84027 (Rat)

Gene Symbol

GSK3B

UniProt

P49841

Additional GSK-3 beta Products

Product Documents for Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.