Human/Mouse/Rat IKK alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3768
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IKK alpha
Gly375-Glu745
Accession # O15111
Gly375-Glu745
Accession # O15111
Specificity
Detects human, mouse, and rat IKK alpha. Does not cross-react with recombinant human (rh) IKK beta, rhIKK gamma, or rhIKK epsilon.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat IKK alpha Antibody
Detection of Human/Mouse/Rat IKK alpha by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat IKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKKa at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IKK alpha in HCT‑116 Human Cell Line.
IKKa was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Sheep Anti-Human/Mouse/Rat IKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat IKK alpha Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HCT-116 human colorectal carcinoma cell line and human peripheral blood mononuclear cells treated with PMA and calcium ionomycin
Sample: Immersion fixed HCT-116 human colorectal carcinoma cell line and human peripheral blood mononuclear cells treated with PMA and calcium ionomycin
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines
Sample: C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using AF3768 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK alpha
I kappaB kinase alpha (IKK alpha) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.
Long Name
IkB Kinase alpha
Alternate Names
CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16
Gene Symbol
CHUK
UniProt
Additional IKK alpha Products
Product Documents for Human/Mouse/Rat IKK alpha Antibody
Product Specific Notices for Human/Mouse/Rat IKK alpha Antibody
For research use only
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