Human/Mouse/Rat IKK gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4365
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Western Blot
Cited:
ELISA Development, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IKK gamma
Met1-Glu411
Accession # O88522
Met1-Glu411
Accession # O88522
Specificity
Detects endogenous human, mouse, and rat IKK gamma in Western blot.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat IKK gamma Antibody
Detection of Mouse and Rat IKK gamma by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for IKK gamma at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat IKK gamma Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblasts embryonic fibroblast, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblasts embryonic fibroblast, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK gamma
I kappaB kinase gamma (IKK gamma) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.
Long Name
IkB Kinase gamma
Alternate Names
FIP3P, IKBKG, IKKAP1, NEMO
Gene Symbol
IKBKG
UniProt
Additional IKK gamma Products
Product Documents for Human/Mouse/Rat IKK gamma Antibody
Product Specific Notices for Human/Mouse/Rat IKK gamma Antibody
For research use only
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