Human/Mouse/Rat ILKAP/PP2C delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4554
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ILKAP
Met1-His392
Accession # Q9H0C8
Met1-His392
Accession # Q9H0C8
Specificity
Detects endogenous human ILKAP in Western blots. In direct ELISAs, this antibody shows weak cross-reactivity with PP2C alpha (4%), PP2C beta (6%), and PP2C gamma (1%).
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Detection of Human/Mouse/Rat ILKAP/PP2C delta by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4554) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ILKAP/PP2C delta at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line
Sample: HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ILKAP/PP2C delta
Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn2+ ions but inhibited by Mg2+ and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.
Long Name
Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C
Alternate Names
PP2C delta
Gene Symbol
ILKAP
UniProt
Additional ILKAP/PP2C delta Products
Product Documents for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Product Specific Notices for Human/Mouse/Rat ILKAP/PP2C delta Antibody
For research use only
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