Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1277F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 188331
Product Specifications
Immunogen
NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Ser32-Asp296
Accession # P78504
Specificity
Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.Detection of Jagged 1 in Rat Cortical Stem Cells by Flow Cytometry.
Rat cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: bEnd.3 Mouse Endothelioma Cell Line and Rat Cortical Stem Cells
Sample: bEnd.3 Mouse Endothelioma Cell Line and Rat Cortical Stem Cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 1
References
- Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
- Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
- Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
- Lai, E.C. (2004) Development 131:965.
- Milner, L.A. et al. (1994) Blood 83:2057.
- Nyfeler, Y. et al. (2005) EMBO J. 24:3504
-
Linheng, L. et al. (1998) Immunity 8:43.
-
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
-
Oda, T. et al. (1997) Nat. Genet. 16:235.
-
Oda, T. et al. (1997) Genomics 43:376.
Alternate Names
CD339, JAG1
Gene Symbol
JAG1
UniProt
Additional Jagged 1 Products
Product Documents for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
Product Specific Notices for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
For research use only
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