Human/Mouse/Rat Keap1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3024
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Ala90-Ile250
Accession # Q14145
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat Keap1 Antibody
Detection of Human/Mouse/Rat Keap1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line . PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Western Blot Shows Human Keap1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Keap1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Keap1 Antibody
Knockout Validated
Western Blot
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line.
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Keap1
Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Keap1 Products
Product Documents for Human/Mouse/Rat Keap1 Antibody
Product Specific Notices for Human/Mouse/Rat Keap1 Antibody
For research use only