Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7305
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Mind Bomb 1/MIB1
Thr365-Gln461
Accession # Q86YT6
Thr365-Gln461
Accession # Q86YT6
Specificity
Detects human, mouse and rat Mind Bomb 1/MIB1 in Western blots and detects recombinant human Mind Bomb 1/MIB1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant MIB2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody
Detection of Human, Mouse, and Rat Mind Bomb 1/MIB1 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Mind Bomb 1/MIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7305) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mind Bomb 1/MIB1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, mouse liver tissue, and rat liver tissue
Sample: 293T human embryonic kidney cell line, mouse liver tissue, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mind Bomb 1/MIB1
MIB1 (Mind bomb 1; also DIP-1 and ZZANK2) is a 110-115 kDa E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non-redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand-expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand-expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006 amino acids (aa) in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc-finger motifs. There is one potential alternative start site at Met84. Over aa 365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.
Long Name
Mindbomb Homolog 1
Alternate Names
DIP1, MIB1, ZZANK2
Gene Symbol
MIB1
UniProt
Additional Mind Bomb 1/MIB1 Products
Product Documents for Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody
Product Specific Notices for Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody
For research use only
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