Human/Mouse/Rat MKK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2515
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 275909
Product Specifications
Immunogen
E. coli-derived recombinant human MKK3
Met1-Ser347
Accession # P46734
Met1-Ser347
Accession # P46734
Specificity
Detects human MKK3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MKK6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat MKK3 Antibody
Detection of Human/Mouse/Rat MKK3 by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, TS1 mouse helper T cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3 Monoclonal Antibody (Catalog # MAB2515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. A specific band for MKK3 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MKK3 in HeLa Human Cell Line.
MKK3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat MKK3 Monoclonal Antibody (Catalog # MAB2515) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat MKK3 Antibody
Application
Recommended Usage
Immunocytochemistry
15-30 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, TS1 mouse helper T cell line, and NRK rat normal kidney cell
Sample: MDA-MB-468 human breast cancer cell line, TS1 mouse helper T cell line, and NRK rat normal kidney cell
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: MKK3
Long Name
Mitogen-activated Protein Kinase Kinase 3
Alternate Names
MAP2K3, MEK3, PRKMK3
Gene Symbol
MAP2K3
UniProt
Additional MKK3 Products
Product Documents for Human/Mouse/Rat MKK3 Antibody
Product Specific Notices for Human/Mouse/Rat MKK3 Antibody
The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.
For research use only
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