Human/Mouse/Rat NEDD8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB49361
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 866916
Product Specifications
Immunogen
E. coli-derived recombinant human NEDD8
Leu2-Gly77
Accession # Q15843
Leu2-Gly77
Accession # Q15843
Specificity
Detects human NEDD8 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat NEDD8 Antibody
Detection of Human, Mouse, and Rat NEDD8 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB49361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NEDD8 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NEDD8 in HeLa Human Cell Line.
NEDD8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat NEDD8 Polyclonal Antibody (Catalog # MAB49361) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat NEDD8 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Sample: Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Reviewed Applications
Read 1 review rated 5 using MAB49361 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NEDD8
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Alternate Names
Neddylin, Rub1
Gene Symbol
NEDD8
UniProt
Additional NEDD8 Products
Product Documents for Human/Mouse/Rat NEDD8 Antibody
Product Specific Notices for Human/Mouse/Rat NEDD8 Antibody
For research use only
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