Human/Mouse/Rat NEDD8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4936
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NEDD8
Leu2-Gly77
Accession # Q15843
Leu2-Gly77
Accession # Q15843
Specificity
Detects human, mouse, and rat NEDD8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than2% cross‑reactivity with recombinant human NEDD9 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat NEDD8 Antibody
NEDD8 in Human Skeletal Muscle.
NEDD8 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using 15 µg/mL Sheep Anti-Human/Mouse/Rat NEDD8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4936) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human, Mouse, and Rat NEDD8 by Western Blot.
Western blot shows lysates of Recombinant Human NEDD8, HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NEDD8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4936) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDD8 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat NEDD8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Western Blot
2 µg/mL
Sample: Recombinant Human NEDD8, HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Sample: Recombinant Human NEDD8, HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NEDD8
NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa sequence identity to NEDD8 from both mouse and canine.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Alternate Names
Neddylin, Rub1
Gene Symbol
NEDD8
UniProt
Additional NEDD8 Products
Product Documents for Human/Mouse/Rat NEDD8 Antibody
Product Specific Notices for Human/Mouse/Rat NEDD8 Antibody
For research use only
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