Human/Mouse/Rat Neurogranin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7947
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Neurogranin
Met1-Asp78
Accession # Q92686
Met1-Asp78
Accession # Q92686
Specificity
Detects human, mouse, and rat Neurogranin Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Neurogranin Antibody
Detection of Human, Mouse, and Rat Neurogranin by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue, human brain (motor cortex) tissue, and rat brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Neurogranin at approximately 14-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Neurogranin in Rat Brain.
Neurogranin was detected in perfusion fixed frozen sections of rat brain (hippocampus) using Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7947) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to pyramidal neurons in the hippocampus. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Detection of Human, Mouse, and Rat Neurogranin by Simple WesternTM.
Simple Western lane view shows lysates of human brain (motor cortex), mouse brain, and rat brain, loaded at 0.2 mg/mL. A specific band was detected for Neurogranin at approximately 12 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7947) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.Applications for Human/Mouse/Rat Neurogranin Antibody
Application
Recommended Usage
ELISA
This antibody functions as a human Neurogranin ELISA detection antibody when paired with Catalog # MAB79473
Immunohistochemistry
0.5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (hippocampus)
Sample: Perfusion fixed frozen sections of rat brain (hippocampus)
Simple Western
20 µg/mL
Sample: Human brain (motor cortex), mouse brain, and rat brain
Sample: Human brain (motor cortex), mouse brain, and rat brain
Western Blot
0.5 µg/mL
Sample: Mouse brain (cortex) tissue, human brain (motor cortex) tissue, and rat brain (cortex) tissue
Sample: Mouse brain (cortex) tissue, human brain (motor cortex) tissue, and rat brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurogranin
Alternate Names
hng, NRGN, RC3
Gene Symbol
NRGN
UniProt
Additional Neurogranin Products
Product Documents for Human/Mouse/Rat Neurogranin Antibody
Product Specific Notices for Human/Mouse/Rat Neurogranin Antibody
For research use only
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