Human/Mouse/Rat p38 gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1347
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 212464
Product Specifications
Immunogen
E. coli-derived recombinant human p38 gamma
Ala7-Leu367
Accession # P53778
Ala7-Leu367
Accession # P53778
Specificity
Detects human, mouse, and rat p38 gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant p38 alpha, p38 beta, or p38 delta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat p38 gamma Antibody
Detection of Human/Mouse/Rat p38 gamma by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38 gamma, p38d and p38a (2 ng/lane) were included. A specific band for native p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
p38 gamma in MCF‑7 and MOLT‑4 Human Cell Lines.
p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control, right panel) using Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in MCF-7 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and Rat p38 gamma by Simple WesternTM.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for p38 gamma at approximately 46 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat p38 gamma Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Simple Western
10 µg/mL
Sample: C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line
Sample: C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line
Sample: C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB1347 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 gamma
Additional p38 gamma Products
Product Documents for Human/Mouse/Rat p38 gamma Antibody
Product Specific Notices for Human/Mouse/Rat p38 gamma Antibody
For research use only
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