Human/Mouse/Rat p53 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1355

R&D Systems, part of Bio-Techne
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BAF1355
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Detection, Proximity Ligation Assay (PLA), Scintillation Proximity Assay

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all p53 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637

Specificity

Detects human, mouse, and rat p53.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse/Rat p53 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human p53
Recombinant Mouse p53
Recombinant Rat p53
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for Human/Mouse/Rat p53 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p53 Biotinylated Antibody

For research use only

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