Human/Mouse/Rat p53 Antibody

Detection of Human p53 by Simple Western^TM.

Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human p53 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human p53 by Knockdown Validated

Overexpression of Survivin in glioma cells affects cell cycle and proliferation in a p53 dependent manner. a Western blot analysis showing cell lysates from U87-MG and U87-MGshp53 after transduction with Survivin and control vectors. The membranes were blotted with anti-p53 (53 kDa), anti-p53s15 (53 kDa), anti-p21waf/cip (21 kDa), anti-gamma H2AX (16 kDa), anti-cyclin D1 (37 kDa) and E (42 kDa singlets +50 kDa doublets) antibodies and the signals comparing control and Survivin cells were measured. The relative band density (fold increase) obtained from the densitometric analysis and normalized to the corresponding control is depicted. alpha-actin (42 kDa) was used as an internal loading control. b Representative BrdU-incorporation assays of U87-MG (left panel) and U87-MGshp53 cells (right panel) with overexpression of Survivin (bottom) compared to the empty vector-transduced controls (top). The overall amount of BrdU-positive fractions as well as the relative levels of BrdU+ and BrdU− cells in fractions with DNA contents of 2n, 4n, 8n and >8n are depicted. c Proliferation index of the U87-MG and U87-MGshp53 cells transduced with Survivin and empty vector controls. Depicted are mean values ± SD. **p < 0.01. d BrdU-incorporation in U87-MG and U87-MGshp53 cell fractions with DNA content >4n. Depicted are mean values ± SD. **p < 0.01. All data were collected 72 h after transduction of cells Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3932-y), licensed under a CC-BY license. Not internally tested by R&D Systems.