Human/Mouse/Rat Peroxiredoxin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3488
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 1
Met1-Lys199
Accession # Q06830
Met1-Lys199
Accession # Q06830
Specificity
Detects human, mouse and rat Peroxiredoxin 1 in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Peroxiredoxin 3 or 4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 1 Antibody
Detection of Human/Mouse/Rat Peroxiredoxin 1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line . PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human Peroxiredoxin 1 by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (PC-3) (NBP2-49856), and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Western Blot Shows Human Peroxiredoxin 1 Specificity Using Knockout Cell Line.
Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.Applications for Human/Mouse/Rat Peroxiredoxin 1 Antibody
Application
Recommended Usage
Knockout Validated
Peroxiredoxin 1 is specificlly detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line.
Simple Western
2-10 µg/mL
Sample: Exosome Standard (HT-29) (Catalog # NBP3-11685), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: Exosome Standard (HT-29) (Catalog # NBP3-11685), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Western Blot
0.2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 1
Human Peroxiredoxin 1 (Prx-1 or PRDX1; also Thioredoxin Peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length, and has two catalytic cysteines, one at Cys52, and a second at Cys173. Prx-1 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms. One shows a premature truncation after aa 171, while the second shows a deletion of aa 21 - 121. Human Prx-1 shows 96% and 98% amino acid identity to mouse and rat Prx-1, respectively.
Alternate Names
NKEF-A, PRDX1, PRX1, TDPX2
Gene Symbol
PRDX1
UniProt
Additional Peroxiredoxin 1 Products
Product Documents for Human/Mouse/Rat Peroxiredoxin 1 Antibody
Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 1 Antibody
For research use only
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