Human/Mouse/Rat PKA C Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4175
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rabbit
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 485013
Product Specifications
Immunogen
E. coli-derived recombinant human PKA C gamma
Met1-Phe351
Accession # ABM84853
Met1-Phe351
Accession # ABM84853
Specificity
Detects human PKA C alpha, C beta, and C gamma as well as endogenous human, mouse, and rat PKA C isoforms at 39-42 kDa using Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat PKA C Pan Specific Antibody
Detection of Human/Mouse/Rat PKA C by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat PKA C Pan Specific Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA C (pan)
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit
Alternate Names
CAFD1, PKACA, PPNAD4
UniProt
Additional PKA C (pan) Products
Product Documents for Human/Mouse/Rat PKA C Pan Specific Antibody
Product Specific Notices for Human/Mouse/Rat PKA C Pan Specific Antibody
For research use only
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