Human/Mouse/Rat PKA RI beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4177
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKA RI beta
Met1-Val381
Accession # P31321
Met1-Val381
Accession # P31321
Specificity
Detects human, mouse, and rat PKA RI beta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKA RI beta Antibody
Detection of Human PKA RI beta by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKA RI beta by Western Blot.
Western blot shows recombinant human PKA RIa, PKA RI beta, and PKA RII beta (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/ Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKA RI beta by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKA RI beta at approximately 56 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat PKA RI beta Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4177 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA RI beta
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, alpha and beta. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
Long Name
cAMP-dependent Protein Kinase Regulatory Type I beta
Alternate Names
PRKAR1B, RIbeta
Gene Symbol
PRKAR1B
UniProt
Additional PKA RI beta Products
Product Documents for Human/Mouse/Rat PKA RI beta Antibody
Product Specific Notices for Human/Mouse/Rat PKA RI beta Antibody
For research use only
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