Human/Mouse/Rat PKN2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5686

R&D Systems, part of Bio-Techne
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MAB5686
MAB5686-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 509105

View all PKN2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKN2
Asp18-Asn207
Accession # Q16513

Specificity

Detects endogenous human, mouse and rat PKN2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat PKN2 Antibody

Detection of Human/Mouse/Rat PKN2 antibody by Western Blot.

Detection of Human/Mouse/Rat PKN2 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKN2 Monoclonal Antibody (Catalog # MAB5686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PKN2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKN2

Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Entrez Gene IDs

5586 (Human); 109333 (Mouse); 207122 (Rat)

Gene Symbol

PKN2

UniProt

Q16513

Additional PKN2 Products

Product Documents for Human/Mouse/Rat PKN2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PKN2 Antibody

For research use only

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