Human/Mouse/Rat PP1 Inhibitor-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4719
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Simple Western, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PP1 Inhibitor-2
Met1-Ser205
Accession # P41236
Met1-Ser205
Accession # P41236
Specificity
Detects human, mouse and rat PP1 Inhibitor-2 in Western blots. In Western blots, no cross-reactivity with recombinant human PP1 Inhibitor-1 or DARPP-32 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PP1 Inhibitor-2 Antibody
Detection of Human/Mouse/Rat PP1 Inhibitor-2 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 Inhibitor-2 at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human PP1 Inhibitor-2 by Simple WesternTM.
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PP1 Inhibitor-2 at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat PP1 Inhibitor-2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and mouse spleen tissue
Sample: Raji human Burkitt's lymphoma cell line and mouse spleen tissue
Reviewed Applications
Read 2 reviews rated 4.5 using AF4719 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP1 Inhibitor-2
Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size.
Long Name
Protein Phosphatase 1 Inhibitor 2
Alternate Names
IPP2, PP1 Inhibitor2, PPP1R2
Gene Symbol
PPP1R2
UniProt
Additional PP1 Inhibitor-2 Products
Product Documents for Human/Mouse/Rat PP1 Inhibitor-2 Antibody
Product Specific Notices for Human/Mouse/Rat PP1 Inhibitor-2 Antibody
For research use only
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