Human/Mouse/Rat Rheb Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3426
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 344912
Product Specifications
Immunogen
E. coli-derived recombinant human Rheb
Met1-Met184
Accession # AAH66307
Met1-Met184
Accession # AAH66307
Specificity
Detects human, mouse, and rat Rheb.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Rheb Antibody
Detection of Human/Mouse/Rat Rheb by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Rheb in Ramos Human Cell Line.
Rheb was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Rheb in NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative).
Rheb was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative) using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Rheb Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative)
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative)
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rheb
Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.
Long Name
Ras Homolog Enriched In Brain
Alternate Names
Rheb2
Gene Symbol
RHEB
UniProt
Additional Rheb Products
Product Documents for Human/Mouse/Rat Rheb Antibody
Product Specific Notices for Human/Mouse/Rat Rheb Antibody
For research use only
Loading...
Loading...
Loading...
Loading...