Human/Mouse/Rat RIPK1/RIP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3585
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 334640
Product Specifications
Immunogen
E. coli-derived recombinant human RIPK1/RIP1
Met1-Asn671
Accession # Q13546
Met1-Asn671
Accession # Q13546
Specificity
Detects human, mouse and rat RIPK1/RIP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat RIPK1/RIP1 Antibody
Detection of Human/Mouse/Rat RIPK1/RIP1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK1/RIP1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
RIPK1/RIP1 in MCF‑7 Human Cell Line.
RIPK1/RIP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human RIPK1/RIP1 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK1/RIP1 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Applications for Human/Mouse/Rat RIPK1/RIP1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Knockout Validated
RIPK1/RIP1
is specifically detected in MCF‑7 human breast cancer cell line parental cell line but is not detectable in
RIPK1/RIP1 knockout MCF‑7 cell line.
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line
Sample: Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line
Reviewed Applications
Read 2 reviews rated 5 using MAB3585 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK1/RIP1
Receptor-Interacting Protein 1 (RIP1, also known as RIPK1) is a 671 amino acid (aa) 75 kDa protein that contains an N-terminal protein kinase domain, a C-terminal death domain, and a unique internal region called the intermediate domain. RIP1 is a serine/threonine protein kinase and is constitutively expressed in many tissues. RIP1 interacts with the cytoplasmic death domain of FAS and TNF receptors and is an important element in the signal transduction machinery that mediates apoptosis. RIP1 has been shown to interact with a number of proteins including TRADD, TRAF1, TRAF2, and TRAF3, to form larger signaling complexes. These complexes, in turn, activate specific signaling cascades, such as NF kappaB. RIP1 also interacts through the C-terminal RIP homotypic interaction motif (RHIM) of TRIF in TLR3 dependent activation of NF kappaB.
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1
Alternate Names
RIP, RIP1
Gene Symbol
RIPK1
UniProt
Additional RIPK1/RIP1 Products
Product Documents for Human/Mouse/Rat RIPK1/RIP1 Antibody
Product Specific Notices for Human/Mouse/Rat RIPK1/RIP1 Antibody
For research use only
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