Human/Mouse/Rat SNAP25 Antibody

Detection of Human and Rat SNAP25 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, H4 human neuroglioma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for SNAP25 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SNAP25 in SH-SY5Y Human Cell Line.

SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (0695/50) prior to immersion fixation. SNAP25 was detected using a Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Sheep Affinity-purified IgG Secondary Antibody (red; NL010). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SNAP25 in PC-12 Rat Adrenal Pheochromocytoma Cells.

PC-12 rat adrenal pheochromocytoma cells were cultured overnight in the presence of 50 ng/mL of Recombinant Rat b-NGF (556-NG) prior to immersion fixation. SNAP25 was detected using a Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Sheep Affinity-purified IgG Secondary Antibody (red; NL010). The cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.