Human/Mouse/Rat Syntaxin 8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5448
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Syntaxin 8
Met1-Gly215
Accession # Q9UNK0
Met1-Gly215
Accession # Q9UNK0
Specificity
Detects human, mouse, and rat Syntaxin 8 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Syntaxin 8 Antibody
Detection of Human, Mouse, and Rat Syntaxin 8 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Syntaxin 8 by Western Blot.
Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 8 in Human Brain.
Syntaxin 8 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat Syntaxin 8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 3 using AF5448 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 8
Syntaxin 8 (STX8) is a 28 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in Golgi, endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX8 is involved in endosomal homotypic fusion events as well as protein trafficking. Human STX8 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 236 amino acids (aa) in length. It contains a coiled-coil region (aa 42‑65), a t-SNARE domain (aa 152‑207) that is likely involved in protein-protein interactions, and a short, four amino acid C-terminal luminal sequence. Over aa 1‑215, human STX8 shares 92% aa identity with mouse STX8.
Alternate Names
CARB, STX8
Gene Symbol
STX8
UniProt
Additional Syntaxin 8 Products
Product Documents for Human/Mouse/Rat Syntaxin 8 Antibody
Product Specific Notices for Human/Mouse/Rat Syntaxin 8 Antibody
For research use only
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