Human/Mouse/Rat TRAF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3277
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Knockout Validated, Western Blot
Cited:
Functional Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 324522
Product Specifications
Immunogen
E. coli-derived recombinant human TRAF-2
Met1-Leu501
Accession # Q12933
Met1-Leu501
Accession # Q12933
Specificity
Detects human, mouse, and rat TRAF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TRAF-1, -3, -4, -5, or -6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat TRAF-2 Antibody
Detection of Human/Mouse/Rat TRAF-2 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, SW3T3 mouse contact inhibited fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TRAF-1, -2, -3, -4, -5, and -6 (2 ng/lane) were included. A specific band for TRAF-2 was detected at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
TRAF‑2 in HeLa Human Cell Line.
TRAF-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human TRAF‑2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRAF-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRAF-2 at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat TRAF-2 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Knockout Validated
TRAF‑2
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
TRAF‑2 knockout HEK293T cell line.
Western Blot
0.25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, SW3T3 mouse contact inhibited fibroblast cell line, and L6 rat myoblast cell line
Sample: Raji human Burkitt's lymphoma cell line, SW3T3 mouse contact inhibited fibroblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAF-2
Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-2, also known as TNF Receptor-Associated Protein (TRAP), is a 501 amino acid, 56 kDa protein which interacts with the cytoplasmic domain of TNFR1, TNFR2 and CD40 to mediate the activation of NF-kappa-B and AP-1 family of transcription factors. TRAF-2 can self-associate as well as form heterodimers with TRAF-1.
Long Name
TNF Receptor-Associated Factor 2
Alternate Names
TRAF2, TRAP3
Gene Symbol
TRAF2
UniProt
Additional TRAF-2 Products
Product Documents for Human/Mouse/Rat TRAF-2 Antibody
Product Specific Notices for Human/Mouse/Rat TRAF-2 Antibody
For research use only
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