Human/Mouse/Rat VAMP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5136
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human VAMP-2
Met1-Lys94
Accession # P63027
Met1-Lys94
Accession # P63027
Specificity
Detects human, mouse, and rat VAMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) VAMP-1 is observed and less than 2% cross-reactivity with rhVAMP-3 and rhVAMP-7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat VAMP-2 Antibody
Detection of Human, Mouse, and Rat VAMP‑2 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
VAMP-2 in Rat Spinal Cord.
Vesicle-Associate Membrane Protein 2 (VAMP-2) was detected in perfusion-fixed frozen sections of rat spinal cord using a Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). The cell nuclei were counterstained with DAPI (blue). VAMP-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat VAMP-2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord
Sample: Perfusion fixed frozen sections of rat spinal cord
Western Blot
1 µg/mL
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAMP-2
Long Name
Vesicle-Associated Membrane Protein 2
Alternate Names
SYB2, VAMP2
Gene Symbol
VAMP2
UniProt
Additional VAMP-2 Products
Product Documents for Human/Mouse/Rat VAMP-2 Antibody
Product Specific Notices for Human/Mouse/Rat VAMP-2 Antibody
For research use only
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