HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using IC5078P in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: RelA/NFkB p65

RelA p65 (v-rel reticuloendotheliosis viral oncogene homolog A) is a 65 kDa member of the NF kappaB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NF kappaB transcription factor, but dimers with it or other family members can also occur. Upon activation, RelA p65 forms an heterotetramer and moves into the nucleus where it binds to specific DNA sequences. An alternatively spliced isoform that lacks amino acids (aa) 222‑231 (p65 delta) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential ser/thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with I kappaB inhibitors.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human)

Gene Symbol

RELA

UniProt

Q04206

Additional RelA/NFkB p65 Products

Product Documents for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC5078P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Detection of RelA/NF kappaB p65 in HeLa Human Cell Line by Flow Cytometry.