Human/Mouse RNA Polymerase II/POLR2A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6160
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Control
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RNA Polymerase II/POLR2A
Leu15-Leu354
Accession # P24928
Leu15-Leu354
Accession # P24928
Specificity
Detects human and mouse RNA Polymerase II/POLR2A in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse RNA Polymerase II/POLR2A Antibody
Detection of Human and Mouse RNA Polymerase II/-POLR2A by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and HT-2 mouse T cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse RNA Polymerase II/-POLR2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for RNA Polymerase II/-POLR2A at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse RNA Polymerase II/POLR2A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, and HT‑2 mouse T cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, and HT‑2 mouse T cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RNA Polymerase II/POLR2A
Long Name
DNA-directed RNA Polymerase II Subunit RPB1
Alternate Names
POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2
Gene Symbol
POLR2A
UniProt
Additional RNA Polymerase II/POLR2A Products
Product Documents for Human/Mouse RNA Polymerase II/POLR2A Antibody
Product Specific Notices for Human/Mouse RNA Polymerase II/POLR2A Antibody
For research use only
Loading...
Loading...
Loading...
Loading...