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Human/Mouse Serpin F1/PEDF Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1177

R&D Systems, part of Bio-Techne
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AF1177
AF1177-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Agonist Activity, Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin F1/PEDF
Asp44-Pro418
Accession # P36955

Specificity

Detects human Serpin F1/PEDF in direct ELISAs. Detects human and mouse Serpin F1/PEDF in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Serpin F1/PEDF Antibody

Detection of Human Serpin F1/PEDF antibody by Western Blot.

Detection of Human Serpin F1/PEDF by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin F1/PEDF antibody by Western Blot.

Detection of Mouse Serpin F1/PEDF by Western Blot.

Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin F1/PEDF antibody in Human Kidney by Immunohistochemistry (IHC-P).

Serpin F1/PEDF in Human Kidney.

Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Serpin F1/PEDF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human kidney

Simple Western

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.2-2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, human liver tissue, human breast tissue, and P19 mouse embryonal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Alternate Names

EPC-1, PEDF

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Gene Symbol

SERPINF1

UniProt

Additional Serpin F1/PEDF Products

Product Documents for Human/Mouse Serpin F1/PEDF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Serpin F1/PEDF Antibody

For research use only

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