Human Serpin F1/PEDF Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1177
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
recombinant human Serpin F1/PEDF
Specificity
Detects human Serpin F1/PEDF in Western blots. In this format, approximately 40% cross-reactivity with recombinant mouse Serpin F1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Serpin F1/PEDF Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin F1/PEDF
Sample: Recombinant Human Serpin F1/PEDF
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F1/PEDF
Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387-411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44-418, human serpin F1 shares 87% aa identity with mouse serpin F1.
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
Additional Serpin F1/PEDF Products
Product Documents for Human Serpin F1/PEDF Biotinylated Antibody
Product Specific Notices for Human Serpin F1/PEDF Biotinylated Antibody
For research use only
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